Diminished CD103 (aEb7) Expression on Resident T cells from the Female Genital Tract of HIV-positive women

David C. Moylan, Paul A. Goepfert, Mirjam-Colette Kempf, Michael S. Saag, Holly E. Richter, Jiri Mestecky, Steffanie Sabbaj

Abstract


Background:Tissue resident memory T cells (TrM) provide an enhanced response against infection at mucosal surfaces, yet their function has not been extensively studied in humans, including the female genital tract (FGT).

Methods: Using polychromatic flow cytometry, we studied TrM cells, defined as CD62L-CCR7-CD103+CD69+ CD4+ and CD8+ T cells in mucosa-derived T cells from healthy and HIV-positive women.

Results: We demonstrate that TrM are present in the FGT of healthy and HIV-positive women. The expression of the mucosal retention receptor, CD103, from HIV-positive women was reduced compared to healthy women and was lowest in women with CD4 counts < 500 cells/mm3. Furthermore, CD103 expression on mucosa-derived CD8+ T cells correlated with antigen-specific IFN-γ production by mucosal CD4+ T cells and was inversely correlated with T-bet from CD8+CD103+ mucosa-derived T cells.

Conclusions: These data suggest that CD4+ T cells, known to be impaired during HIV-1 infection and necessary for the expression of CD103 in murine models, may play a role in the expression of CD103 on resident T cells from the human FGT.


Keywords


Tissue Resident Memory T cells; female genital tract; HIV-1; CD103; mucosal immunity; CD8+ T cells; CD4 T cells

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References


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